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jurkat human leukemic t cell line  (ATCC)


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    ATCC jurkat human leukemic t cell line
    Jurkat Human Leukemic T Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+acute+leukemic+jurkat+t+cells/Neo+Jurkat%3B+Acute+T+Cell+Leukemia%3B+Human/pm41539832-63-1-10
    Average 95 stars, based on 392 article reviews
    jurkat human leukemic t cell line - by Bioz Stars, 2026-09
    95/100 stars

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    Transplantation Assay:

    Article Title: Resident phagocytes promote non–cell-autonomous fragmentation of apoptotic cells
    Article Snippet: Murine thymocyte suspensions were maintained in RPMI medium containing 10% (v/v) FBS (Scientifix), penicillin (50 U/ml), and streptomycin (50 μg/ml), at 37°C in 5% CO 2 . .. For zebrafish transplantation experiments, human acute leukemic Jurkat T cells (clone E6-1, American Type Culture Collection, Bethesda, MD, USA) obtained from A. Jordan (Institute of Molecular Biology of Barcelona) were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal calf serum, penicillin (100 U/ml) and streptomycin (100 mg/ml), sodium pyruvate (110 mg/liter), and 2 mM l -glutamine, at 37°C in 5% CO 2 . .. Cells were treated with 10 μM Raptinal for 4 hours, washed in DMEM (DMEM/F-12 with l -glutamine and Hepes, Sigma-Aldrich), and stained with FM 4-64FX (5 μg/ml; Thermo Fisher Scientific) for 5 min.

    Article Title: Resident phagocytes promote non-cell-autonomous fragmentation of apoptotic cells.
    Article Snippet: Murine thymocyte suspensions were maintained in RPMI medium containing 10% (v/v) FBS (Scientifix), penicillin (50 U/ml), and streptomycin (50 μg/ml), at 37°C in 5% CO2. .. For zebrafish transplantation experiments, human acute leukemic Jurkat T cells (clone E6- 1, American Type Culture Collection, Bethesda, MD, USA) obtained from A. Jordan (Institute of Molecular Biology of Barcelona) were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal calf serum, penicillin (100 U/ml) and streptomycin (100 mg/ml), sodium pyruvate (110 mg/liter), and 2 mM l- glutamine, at 37°C in 5% CO2. .. Cells were treated with 10 μM Raptinal for 4 hours, washed in DMEM (DMEM/F- 12 with l- glutamine and Hepes, Sigma- Aldrich), and stained with FM 4- 64FX (5 μg/ml; Thermo Fisher Scientific) for 5 min.



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    jurkat human leukemic t cell line  (ATCC)
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    ATCC jurkat human leukemic t cell line
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    CD5L promotes chemotaxis of natural killer T (NKT) cells. A: Schematics of the Transwell assay. B: Transwell assay performed with primary liver immune cells in the insert with or without 2000 ng/mL CD5L in the bottom chamber. No CD5L wells contain media and 0.1% fetal bovine serum (FBS). Cells recovered in the bottom were analyzed for immune cell types using flow cytometry and reported as percentage of cell distributions. C: Transwell assay performed with primary liver immune cells in the insert with or without liver macrophage (LMac) in the bottom chamber. No LMac wells contain media and 0.1% FBS. Cells recovered in the bottom were analyzed for immune cell types using flow cytometry and reported as percentage of cell distributions. Each dot is one mouse. Data are not significant (NS), P = 0.057. D: Transwell assay performed with <t>Jurkat</t> cells in the insert and 2% FBS with indicated doses of CD5L in the bottom chamber. E: Transwell assay performed with primary liver immune cells in the insert and LMac in the bottom chamber. Treatment with or without CD5L neutralizing antibody or CD36 inhibitor SMS121 is compared with vehicle treatment (0.1% FBS). F: Schematic representation of the overall function of CD5L in the liver: CD5L produced by LMacs facilitates lipid uptake in hepatocytes and stellate cells and regulates the migration of NKT cells. G: Correlation of CD5L with Nk1.1, a marker of NKT in liver injury models. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001; # P < 0.05 only detected in experimental group.
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    CD5L promotes chemotaxis of natural killer T (NKT) cells. A: Schematics of the Transwell assay. B: Transwell assay performed with primary liver immune cells in the insert with or without 2000 ng/mL CD5L in the bottom chamber. No CD5L wells contain media and 0.1% fetal bovine serum (FBS). Cells recovered in the bottom were analyzed for immune cell types using flow cytometry and reported as percentage of cell distributions. C: Transwell assay performed with primary liver immune cells in the insert with or without liver macrophage (LMac) in the bottom chamber. No LMac wells contain media and 0.1% FBS. Cells recovered in the bottom were analyzed for immune cell types using flow cytometry and reported as percentage of cell distributions. Each dot is one mouse. Data are not significant (NS), P = 0.057. D: Transwell assay performed with Jurkat cells in the insert and 2% FBS with indicated doses of CD5L in the bottom chamber. E: Transwell assay performed with primary liver immune cells in the insert and LMac in the bottom chamber. Treatment with or without CD5L neutralizing antibody or CD36 inhibitor SMS121 is compared with vehicle treatment (0.1% FBS). F: Schematic representation of the overall function of CD5L in the liver: CD5L produced by LMacs facilitates lipid uptake in hepatocytes and stellate cells and regulates the migration of NKT cells. G: Correlation of CD5L with Nk1.1, a marker of NKT in liver injury models. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001; # P < 0.05 only detected in experimental group.

    Journal: The American Journal of Pathology

    Article Title: Characterizing Kupffer Cell Production of CD5 Antigen-Like and Its Function on Regulating Migration of Natural Killer T Cells

    doi: 10.1016/j.ajpath.2025.06.003

    Figure Lengend Snippet: CD5L promotes chemotaxis of natural killer T (NKT) cells. A: Schematics of the Transwell assay. B: Transwell assay performed with primary liver immune cells in the insert with or without 2000 ng/mL CD5L in the bottom chamber. No CD5L wells contain media and 0.1% fetal bovine serum (FBS). Cells recovered in the bottom were analyzed for immune cell types using flow cytometry and reported as percentage of cell distributions. C: Transwell assay performed with primary liver immune cells in the insert with or without liver macrophage (LMac) in the bottom chamber. No LMac wells contain media and 0.1% FBS. Cells recovered in the bottom were analyzed for immune cell types using flow cytometry and reported as percentage of cell distributions. Each dot is one mouse. Data are not significant (NS), P = 0.057. D: Transwell assay performed with Jurkat cells in the insert and 2% FBS with indicated doses of CD5L in the bottom chamber. E: Transwell assay performed with primary liver immune cells in the insert and LMac in the bottom chamber. Treatment with or without CD5L neutralizing antibody or CD36 inhibitor SMS121 is compared with vehicle treatment (0.1% FBS). F: Schematic representation of the overall function of CD5L in the liver: CD5L produced by LMacs facilitates lipid uptake in hepatocytes and stellate cells and regulates the migration of NKT cells. G: Correlation of CD5L with Nk1.1, a marker of NKT in liver injury models. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001; # P < 0.05 only detected in experimental group.

    Article Snippet: HepG2, human T-cell leukemic Jurkat cells, and Raw264.7 cells were obtained from ATCC (Manassas, VA).

    Techniques: Chemotaxis Assay, Transwell Assay, Flow Cytometry, Produced, Migration, Marker

    Journal: Cell Reports

    Article Title: Caspase cleavage and nuclear retention of the energy sensor AMPK-α1 during apoptosis

    doi: 10.1016/j.celrep.2022.110761

    Figure Lengend Snippet:

    Article Snippet: Human leukemic Jurkat , ATCC , TIB-152; RRID:CVCL_0367.

    Techniques: Virus, Recombinant, Saline, Protease Inhibitor, Transfection, Expressing, Extraction, Membrane, Integrity Assay, Mutagenesis, Single Cell Gel Electrophoresis, Control, Plasmid Preparation, Bacteria, Software